Quantification of free citrulline by microdiffusion method
Abstract
Citrulline is a non-proteinogenic amino acid that is involved both in free form and in peptides (peptidylcitrulline) in many important metabolic processes. Quantification of citrulline is an analytical technology in demand in various fields. Unfortunately, not all currently available methods for the quantitative determination of citrulline have satisfactory analytical performance and/or availability. For this reason, the development of new methods for the quantitative determination of this metabolite is an urgent task. The proposed modified method is based on the microdiffusion distillation of ammonia, an accessible and easily implemented approach that makes it possible to accurately determine the citrulline content in solutions and biological samples.
References
1. György B., Tóth E., Tarcsa E., Falus A., Buzás E. I. Citrullination: a posttranslational modification in health and disease // Int. J. Biochem. Cell Biol. 2006. Vol. 38, № 10. P. 1662–1677.
2. Slade D. J., Subramanian V., Fuhrmann J., Thompson P. R. Chemical and biological methods to detect post-translational modifications of arginine // Biopolymers. 2014. Vol. 101, № 2. P. 133–143.
3. Vossenaar E. R., Zendman A. J., van Venrooij W. J., Pruijn G. J. PAD, a growing family of citrullinating enzymes: genes, features and involvement in disease // BioEssays. 2003. Vol. 25, № 11. P. 1106–1118.
4. Glant T. T., Ocsko T., Markovics A., Szekanecz Z., Katz R. S., Rauch T. A., Mikecz K. Characterization and Localization of Citrullinated Proteoglycan Aggrecan in Human Articular Cartilage // PLoS One. 2016. Vol. 11, № 3. P. 76–98.
5. Cantaert T., De Rycke L., Bongartz T., Matteson E. L., Tak P. P., Nicholas A. P., Baeten D. Citrullinated proteins in rheumatoid arthritis: crucial... but not sufficient! // Arthritis Rheum. 2006. Vol. 54, № 11. P. 3381–3389.
6. Майстер А. Биохимия аминокислот / под ред. и с предисл. А. Е. Браунштейн ; пер. с англ. Г. Я. Виленкина. М. : Иностр. лит., 1961. 530 с.
7. Силакова А. И., Труш Г. П., Явилякова А. Микрометод определения аммиака и глутамина в тканевых трихлоруксусных экстрактах // Вопросы медицинской химии. 1962. Т. 8, № 5. С. 538–544.
Review
For citations:
Noskova V.S. Quantification of free citrulline by microdiffusion method. Title in english. 2021;16(2):25-29. (In Russ.)
Views:
163
Email this article 