Immunohistochemical endometrial predictors of successful implantation and live birth in assisted reproductive technology programs
Abstract
The aim of the study was to develop a method for predicting blastocyst implantation and live birth in assisted reproductive technology programs in women of late reproductive age with tubal-peritoneal infertility based on the determination of immunohistochemical determinants of the endometrial pattern.
Materials and methods. The results of IVF and IVF/ICSI programs using own oocytes were analyzed in 68 patients of older reproductive age with tubal-peritoneal infertility factor. Histological examination of endometrial samples (n=68) was performed on day 7 after ovulation in the cycle preceding
assisted reproductive technologies (ART). The expression of vitamin D (VDR) and HOXA11 receptors in endometrial stromal cells was evaluated by immunohistochemistry.
The results of the study. The expression levels of VDR (<8.7 %) and HOXA11 (<6.1 %) favorable for successful implantation of the blastocyst were determined, live births — VDR (<8.3 %) and HOXA11 (<6.1 %). Mathematical models have been developed that allow predicting implantation (with an index >0.17) and live birth (index >0.19) with high diagnostic accuracy based on an assessment of the expression of the expression variables VDR and HOXA11 stroma.
Conclusion. The effectiveness of using endometrial markers VDR and HOXA11 as potential predictors of the effectiveness of ART programs is confirmed by prognostic models and patents of the Russian Federation.
Keywords
immunohistochemistry,
expression,
vitamin D receptors (VDR),
HOXA11,
prognostic models,
implantation,
live birth
Supporting agencies: The research was carried out with the financial support of the RFBR and the Chelyabinsk Region as part of a scientific project № 20-415-740014
About the Authors
E. G. Chuhnina
South-Urals State Medical University
Russian Federation
Russian Federation
Chelyabinsk
E. L. Kazachkov
South-Urals State Medical University
Russian Federation
Russian Federation
Chelyabinsk
E. E. Voropaeva
South-Urals State Medical University
Russian Federation
Russian Federation
Chelyabinsk
E. A. Kazachkova
South-Urals State Medical University
Russian Federation
Russian Federation
Chelyabinsk
M. E. Luzan
South-Urals State Medical University
Russian Federation
Russian Federation
Chelyabinsk
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Review
For citations:
Chuhnina E.G., Kazachkov E.L., Voropaeva E.E., Kazachkova E.A., Luzan M.E. Immunohistochemical endometrial predictors of successful implantation and live birth in assisted reproductive technology programs. Title in english. 2023;18(2):32-38. (In Russ.)
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